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You are starting with lossy, so you cannot get back what is already gone. You can lose more every time you save a JPG. You could convert to TIFF.
The best method would be to shoot RAW, if your camera supports it. Your camera would have come with software that is capable of converting RAW to something else.
I just looked at the gallery, your D300 does support RAW. I am a Canon person so I cannot help with any specifics on the Nikon software.
You did not even mention the name of what software you are using.
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