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The D300 is a bigger and heavier camera so you really need to handle both to see which feels the more comfortable to you. The D300 is built like a tank and is consequently significantly more durable than the D7000. Both will give you superb images but the D7000 has the edge, particularly at higher ISO. The D300's autofocus is probably the best of any APS-C DSLR particularly for sport, etc, where you need to track the subject.
If you are into video you will need to be comparing the D7000 with the D300s, not the D300.
At the end of the day it is a matter of which camera suits your photography needs best, but neither would disappoint.
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