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I understand that the dof would depend on the aperture, and additionally by the magnification used.
I've spent the last 3/4 of an hour reading reviews and looking at field examples with explanations of macro work captured using the Canon MP-E 65 f2.8 5x1 macro lens. You have to be very patient when setting up and shooting your stacked images, but it transpires that the donkey work of processing those images can be automised using Zerene Stacker, Helicon Focus, Photoshop etc.
You have managed to expose these images really well and without a dedicated macro flash to boot.
My last question then is, given the difficulty that 5x magnification would have with light levels and aperture settings, what was the magnification of the first two images? The reason I ask is to give me some idea how much of your subject could you have filled the frame with these shots, given that you haven't cropped them.
Mind you, looking at that wasp I hope the magnification was x3 or more, because if that was a 1:1 image I wouldn't like to come across that monster in a hurry!
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